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. Author manuscript; available in PMC: 2018 Jul 13.
Published in final edited form as: Exp Hematol. 2017 Apr 27;51:17–24. doi: 10.1016/j.exphem.2017.04.003

Figure 3.

Figure 3

CML basophils express higher levels of CD25 than do basophils obtained from healthy donors. Expression of CD25 on CD123++/CD45+ basophils was analyzed by multicolor flow cytometry using fluorochrome-labeled antibodies against CD45, CD123, and CD25. Cells were obtained from five patients with CP CML (left red bar) and five healthy controls (right blue bar). Expression of CD25 was determined as the MFI and is expressed as the SI according to the following formula: SI = MFI test antibody: MFI isotype-control antibody. *p < 0.05 compared with CD25 expression on normal basophils. MFI = Median fluorescence intensity; SI = staining index.