Figure 4. Cytokine production by colonic lamina propria cells following DSS-induced injury.
Colonic LPL cells were restimulated with media alone, IL-23 or αCD3 overnight and supernatant was analyzed by ELISA or luminex to determine levels of IL-17A, IL-17F, IFNγ and IL-22. (A) Cytokine protein levels detected in supernatants of cLPL isolated from either WT (open bars) and Il-17−/− (closed bars) mice, *p<0.016; **p=0.007; (B) WT and Rag−/− mice, (IL-17A *p=0.0001;**p<0.005), (IFNγ *p<0.048, **p<0.006).