Extended Data Figure 8. miR-221/222 is upregulated in human cells and sepsis patients.

a, b, LPS-induced miRNA expression in undifferentiated (a) or PMA-differentiated (b) human U937 cells (n=3 independent experiments). c, Patient characteristics for data in Fig. 4a. ICU, intensive care unit; APACHE, acute physiology and chronic health evaluation; SAPS, simplified acute physiology score. Categorical variables are given as (n, %) and continuous variables as (median, IQR). d,e Baseline characteristics of patients with decompensated liver disease in the absence or presence of multiple organ failure syndrome (according to the EASL CLIF-C criteria for Acute-on-chronic Liver Failure). Data in table (d) corresponds to PBMC analyses (Fig. 4b–d). Median with interquartiles or frequencies and percentages are shown. P values from Mann-Whitney U test or Fisher’s exact test as appropriate (2-sided). *comparing any infection versus no infection. ** 4/30 (13%) and 1/10 (10%) patients were lost to follow-up within 30 days. Data in table (e) corresponds to monocyte analyses (Fig. 4f). Median with interquartiles or frequencies and percentages are shown. P values from Mann-Whitney U test of Fisher’s exact test as appropriate (2-sided). *comparing any infection versus no infection. ** 1/10 (10%) patients were lost to follow-up within 30 days. f, Correlation between miR-221 and miR-222 levels in patients characterized in (d; n=30 patients). Bivariate nonparametric correlation analysis (Spearman’s rho) was used to identify correlations between variables and p-values. g-j, Linear correlation of miR-222 expression and CRP (g), WBC count (h) creatinine levels (i) or MELD score (j) in samples from the patient cohort described in (d; n=30 patients). Bivariate nonparametric correlation analysis (Spearman’s rho) was used to identify correlations between variables and p-values. For line graphs, mean +/− SEM is plotted.