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. 2018 Jul 2;16(7):e2006333. doi: 10.1371/journal.pbio.2006333

Fig 2. Examples of EMS transformed protists.

Fig 2

(A, B) Corallochytrium limacisporum stably expressing the mCherry fluorescent protein (red) fused to a puromycin resistance protein driven by an endogenous actin promoter (M. Rubio-Brotons, UPF–CSIC, Barcelona, Spain). (C, D) Perkinsus olseni (marine bivalve parasite) expressing a GFP (green) fusion with an exported cell-wall protein (R. Waller, University of Cambridge, United Kingdom). (E, F) The choanoflagellate Salpingoeca rosetta transformed with a plasmid expressing fluorescent proteins that illuminate the cell body (green) and the plasma membrane (magenta) (D. Booth, University of California Berkeley, United States of America). Scale bar = 5 μm, 20 μm, and 5 μm for B, D, and F, respectively. EMS, Experimental Model Systems; GFP, green fluorescent protein; UPF–CSIC, Universitat Pompeu Fabra–Spanish National Research Council.