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. 2018 Jul 2;12(7):e0006608. doi: 10.1371/journal.pntd.0006608

Fig 4. 25D3 status contributes to IL-15 MΦ antimicrobial response.

Fig 4

A) Colocalization of M. leprae (mLEP = red) and cathelicidin (CATH = green) 24 hours PI in IL-15 MΦdifferentiated in the presence of 25D3 for 48 hours. (Original magnification, 400); (6.5x zoom of original magnification). Scale bar = 5μm. IL-15 MΦ were differentiated in increasing concentrations of 25D3 (0 to 10-7M) for 48 hours and infected with M. leprae for B) 24 hours (n = 3), C) 48 hours (n = 5) or D) 120 hours (n = 5) and CAMP mRNA expression was assessed by qPCR. Data is represented as the average fold change in CAMP mRNA +/- SEM relative to IL-15 MΦ differentiated in no 25D3. IL-15 MΦ were differentiated in increasing concentrations of 25D3 (0 to 10-7M) for 48 hours and infected with M. leprae for E) 24 hours (n = 3), F) 48 hours (n = 5) or G) 120 hours (n = 5) and bacteria burden was assessed by qPCR. Data is represented as the average change in bacteria burden +/- SEM relative to IL-15 MΦ differentiated in no 25D3. *P<0.05, **P<0.01, ***P<0.005, ****P<0.001.