Figure 2.

Anti-inflammatory effect of protein kinase CK-1δ inhibitors on the glial primary cultures. Astrocytes (A, C) and microglial cells (B, D) cultures were isolated, plated, and later treated with lipopolysaccharide (LPS, 10 μg/mL) in the presence of the different CK-1δ inhibitors (10 μM) and compound 4 (0.1–20 μM). The production of nitrite was measured by the Griess reaction. Each data point represents the mean ± SD of six replications in three different experiments. ***p < 0.001, statistically significant differences between CK-1δ inhibitors and LPS-treated cultures.