Figure 2.

Gelatin/HA film characteristics: (A) Thickness of the film cross-linked with TGA determined by a confocal microscope using PLL^Rho as a fluorescent labeling agent. (B) SEM picture of the film (left: x,y surface, right: cross section). (C) Co-localization of HA^FITC and PLL^Rho throughout the film thickness determined with a confocal microscope to prove HA homogeneous distribution within the film. (D) Stability of the gelatin/HA film at 37 °C in phosphate-buffered saline (PBS) without poly(ethyleneimine) (PEI) as a first layer after 3 and 7 days.