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. 2017 Mar 15;2(3):918–929. doi: 10.1021/acsomega.6b00502

Figure 8.

Figure 8

Release of IL-4 initially loaded in the gelatin/HA-tyr film to the hydrogel with THP-1 encapsulated in the hydrogel. (A) Metabolic activity of encapsulated THP-1 in gelatin hydrogel with and without IL-4 release from the film. (B, C) Confocal images of THP-1 encapsulated in the hydrogel by DAPI (blue) staining after 21 days with and without IL-4 release. (D) Results of real-time PCR analysis of M1 markers for 3D encapsulated THP-1 cells without or with IL-4 release. The M1 markers are as follows: CD86, IL-6, STAT1, and TNF-α. (E, F) Confocal images of THP-1 encapsulated in the hydrogel with the M1 marker, CD80 (red), and the M2 marker, CD206 (green) and DAPI (blue).