Table 2. IC₅₀ Data of p-Bromobenzamide Replacements of the Scaffold 2 for the Disruption of Cbx7–Compound 1 Complex^a.

^aBinding data determined from the inhibitor concentration needed to half-dissociate the Cbx7–compound 1 complex. Data are the averages of two or three independent experimental trails and expressed as IC₅₀ ± standard error in μM. FP data obtained upon titrating varied concentrations of inhibitors into a fixed concentration of Cbx protein and compound 1. Buffer used in the FP experiments is the same as reported in Table 1. The concentration of compound 1 is 100 nM. The concentrations used for Cbx1, 2, 4, 6, 7, and 8 are 10, 1, 1, 1, 0.4, and 7 μM respectively. NA = not applicable, that is, compounds were not tested.