Table 3. IC₅₀ Data for the Disruption of Cbx7–Compound 1 Complex by N-Alkylated Lysine Replacements Based on Scaffold 2^a.

^aBinding data determined from the inhibitor concentration needed to half-dissociate the complex of Cbx7–compound 1. Data are the averages of two or three independent experimental trails and expressed as IC₅₀ ± standard error in μM. FP data is obtained upon titrating varied concentrations of inhibitors into a fixed concentration of Cbx protein and compound 1. Buffer and concentrations of compound 1 and Cbx protein are as reported in Table 2. NA = not applicable, that is, compounds were not tested. ND = not determined, that is, compounds were tested but because of the factors such as aggregation, IC₅₀ values could not be determined.