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. 2017 Jan 25;2(1):227–235. doi: 10.1021/acsomega.6b00368

Figure 2.

Figure 2

Elution profiles of conjugates on SEC. A 25 μL sample of (a) a mixture of CpG (4.7 μM) and OVA (1.7 μM), (b) 5′ CpG–OVA conjugate (equivalent to 4.7 μM CpG and 1.7 μM OVA), and (c) 3′ CpG–OVA conjugate (equivalent to 4.7 μM CpG and 1.7 μM OVA) was injected onto a Yarra-2000 SEC column equilibrated in phosphate buffer at a flow rate of 0.35 mL/min, and the absorbance was recorded at 260 nm.