Table 6. Primer Sequences Used for Real-Time PCR.
| genea | accession no. | primer sequencesb (5′–3′) | size, bp |
|---|---|---|---|
| Bcl-2 | XM_021099593.1 | F: GCTACTTACTGCCAAAGGGA | 161 |
| R: TTCAGGCGGAGCTGTAAGAG | |||
| Bax | XM_013998624.2 | F: GACGCTGGACTTCCTTCGAG | 334 |
| R: GTGGCCCGAGAGAGGTTTATT | |||
| Fas | NM_213839 | F: TGATGCCCAAGTGACTGACC | 103 |
| R: GCAGAATTGACCCTCACGAT | |||
| caspase-3 | NM_214131.1 | F: GGAATGGCATGTCGATCTGGT | 351 |
| R: ACTGTCCGTCTCAATCCCAC | |||
| caspase-8 | XM_021074714.1 | F: TCTGCGGACTGGATGTGATT | 165 |
| R: TCTGAGGTTGCTGGTCACAC | |||
| caspase-9 | XM_013998997.2 | F: AATGCCGATTTGGCTTACGT | 195 |
| R: CATTTGCTTGGCAGTCAGGTT | |||
| GAPDH | NM_001206359.1 | F: TCGGAGTGAACGGATTTGGC | 147 |
| R: TGCCGTGGGTGGAATCATAC |
a
Bcl-2, B-cell lymphoma-2; Bax, B-cell lymphoma-2-associated X protein; and GAPDH, glyceraldehyde-3-phosphate dehydrogenase.
b
F, forward and R, reverse.