Figure 4.

Post-thaw RBC integrity after freezing using O-aryl-glycoside IRIs in 15% glycerol cryosolutions. (A) Rate-controlled slow-freezing at 1 °C/min to −40 °C followed by −80 °C storage. (B) Rapid-freezing to −80 °C by plunging into solid CO₂ (dry ice). All samples were thawed at 37 °C, and post-thaw RBC hemolysis was determined using the Drabkin’s assay. Results are presented as mean post-thaw % RBC integrity (100 − % hemolysis). The error bars represent SEM. ⧫ indicates compounds with a potent IRI activity.