Table 2. Comparative HisND1–Zn Distances of Mesophilic Ng α-CA, Thermophilic Ssp α-CA and Mutant Ng α-CA.
| 300 K |
350 K |
400 K |
500 K |
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| meso | thermo | mutant | meso | thermo | mutant | meso | thermo | mutant | meso | thermo | mutant | |
| 10 ns | 9.9 | 9.3 | 11.7 | 7.6 | 9.7 | 14.3 | 8.3 | 9.8 | 13.0 | 12.6 | 6.8 | 6.3 |
| 20 ns | 10.2 | 7.2 | 12.3 | 7.7 | 8.5 | 13.9 | 6.4 | 8.2 | 11.2 | 9.8 | 6.7 | 8.8 |
| 30 ns | 11.1 | 11.2 | 11.3 | 8.0 | 12.3 | 13.4 | 10.2 | 10.2 | 10.6 | 10.6 | 14.4 | 18.5 |
| 40 ns | 12.0 | 13.0 | 10.5 | 7.2 | 11.3 | 13.7 | 10.6 | 8.7 | 7.5 | 16.9 | 12.9 | 7.5 |
| 50 ns | 7.4 | 11.4 | 10.5 | 7.3 | 8.4 | 13.2 | 7.9 | 4.5 | 8.1 | 6.9 | 10.7 | 10.4 |
| 60 ns | 8.4 | 7.4 | 11.6 | 10.1 | 8.1 | 13.9 | 7.6 | 8.8 | 7.7 | 15.1 | 16.4 | 13.2 |
| 70 ns | 8.2 | 9.2 | 11.3 | 7.9 | 12.4 | 12.9 | 7.8 | 4.2 | 8.4 | 7.8 | 17.4 | 13.9 |
| 80 ns | 7.5 | 11.9 | 10.7 | 11.5 | 8.1 | 14.0 | 7.4 | 4.0 | 9.0 | 10.1 | 13.6 | 12.6 |
| 90 ns | 8.5 | 10.5 | 11.2 | 6.7 | 7.4 | 13.0 | 9.4 | 11.2 | 9.2 | 14.7 | 20.9 | 10.6 |
| 100 ns | 8.2 | 10.8 | 11.0 | 8.3 | 8.7 | 13.9 | 9.5 | 8.7 | 7.2 | 17.1 | 16.1 | 10.6 |
a
All distances are in Angstrom (Å). HisND1–Zn distances ≤8 Å are in bold.