Table 5. Comparative RMSF of Residues Forming Destabilizing Salt Bridge and the Corresponding Thermophilic Residues.
300 K |
350 K |
400 K |
500 K |
||||||
---|---|---|---|---|---|---|---|---|---|
mesophilic α-CA (M) | thermophilic α-CA (T) | M | T | M | T | M | T | M | T |
Lys102 | Glu99 | 0.1293 | 0.1388 | 0.1632 | 0.1921 | 0.1948 | 0.2436 | 0.4467 | 0.3085 |
Arg104 | Lys101 | 0.0778 | 0.1777 | 0.1173 | 0.2029 | 0.1729 | 0.2314 | 0.3876 | 0.2895 |
Lys132 | Lys129 | 0.2391 | 0.1812 | 0.2182 | 0.1896 | 0.2709 | 0.198 | 0.5366 | 0.329 |
Arg136 | Glu133 | 0.2914 | 0.086 | 0.2896 | 0.0933 | 0.3214 | 0.1142 | 0.7206 | 0.3868 |
Lys165 | Lys162 | 0.2519 | 0.1952 | 0.2491 | 0.2053 | 0.2403 | 0.2226 | 0.7082 | 0.3132 |
Arg166 | Asp163 | 0.3193 | 0.0943 | 0.2513 | 0.1149 | 0.2589 | 0.1266 | 0.6994 | 0.2229 |
Lys168 | Arg165 | 0.1765 | 0.0753 | 0.1638 | 0.166 | 0.1747 | 0.2322 | 0.5154 | 0.2847 |
RMSF values in bold represent comparatively less fluctuation of thermophilic residues corresponding to its mesophilic residues at the respective temperature.