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. 2017 Jul 21;2(7):3792–3805. doi: 10.1021/acsomega.7b00116

Figure 5.

Figure 5

Specific internalization of the FGF2 WT and FGF2-vcMMAE conjugates into the cells expressing FGFR1. Shown are the representative images of internalization of FGF2 WT, KCK-FGF2[C78S/C96S]-(vcMMAE)1, or KCK-FGF2-(vcMMAE)3 into U2OS-R1 cells vs U2OS cells. Equal numbers of U2OS cells stably stained with CellTrace Violet (blue) and U2OS-R1 (nonstained) were grown together and then incubated with 1000 ng/mL FGF2 WT, KCK-FGF2[C78S/C96S]-(vcMMAE)1, or KCK-FGF2-(vcMMAE)3 labeled with DyLight550 (red) at 37 °C for 15 min. The cells were fixed, stained with anti-EEA1 antibody (green), and examined by confocal microscopy. U2OS-R1 cells are marked with a dashed line. The bar corresponds to 10 μm.