Table 1. Kinetic Rate Constants and Dissociation Constants Determined for the Interaction of Wild-Type FGF2 or FGF2 Conjugates with FGFR1c.

analyte	kon × 105 [M–1 s–1]	SE (kon) × 103	koff × 10–4 [s–1]	SE (koff) × 10–6	KD × 10–10 [M]	a	Rmax [nm]	SE (Rmax) × 10–4	kobs [1/s]	χ2 × 10–5
FGF2 WT 80 nM	9.1	4.25	3.51	10.0	3.85	2.43	0.345	4.36	0.0733	2.94
FGF2 WT 40 nM	13.0	5.44	2.86	10.0	2.28		0.341	4.45	0.0506
FGF2 WT 20 nM	19.0	7.89	2.18	9.68	1.15		0.346	4.54	0.0381
KCK-FGF2[C78S/C96S]-(vcMMAE)1 80 nM	5.45	3.51	5.33	16.3	9.77	8.27	0.312	6.85	0.0441	5.31
KCK-FGF2[C78S/C96S]-(vcMMAE)1 40 nM	8.49	5.33	5.80	15.6	6.83		0.324	7.00	0.0345
KCK-FGF2[C78S/C96S]-(vcMMAE)1 20 nM	8.66	6.95	7.11	14.9	8.21		0.343	8.40	0.018
FGF2 WT-(vcMMAE)2 80 nM	6.91	4.41	2.54	15.7	3.67	4.43	0.353	7.37	0.0556	6.56
FGF2 WT-(vcMMAE)2 40 nM	9.16	5.39	3.86	14.8	4.22		0.374	7.69	0.037
FGF2 WT-(vcMMAE)2 20 nM	9.52	7.00	5.14	14.6	5.40		0.38	8.61	0.0195
KCK-FGF2-(vcMMAE)3 80 nM	2.44	1.01	6.61	8.91	27.1	20.8	0.353	5.48	0.0201	3.67
KCK-FGF2-(vcMMAE)3 40 nM	3.09	1.62	6.48	9.69	21.0		0.374	9.81	0.013
KCK-FGF2-(vcMMAE)3 20 nM	2.62	2.61	3.75	9.89	14.3		0.534	38.3	0.00562

^aBold numbers are averages of K_D values from previous column.