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. Author manuscript; available in PMC: 2018 Jul 13.
Published in final edited form as: Nat Protoc. 2018 Mar 29;13(5):819–839. doi: 10.1038/nprot.2017.148
Component Amount Final concentration
End Prep Enzyme Mix 3μL 1×
End Repair Reaction Buffer 10× 6.5μL 1×
Fragmented DNA from Step 28+ H2O 55.5μL 7.7 ng/mL to 15.4 μg/mL
Total Volume 65μL