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. Author manuscript; available in PMC: 2018 Jul 13.
Published in final edited form as: Nat Protoc. 2018 Mar 29;13(5):819–839. doi: 10.1038/nprot.2017.148
Component Amount Final concentration
Mix from step 32 65μL 1×
Blunt/TA Ligase Master Mix 15μL 1×
NEBNext Adaptor for Illumina 2.5μL 0.45μM
Ligation Enhancer 1μL 1×
Total Volume 83.5μL