| Component | Amount | Final concentration |
|---|---|---|
| BrdU IP library from step 55 | 15μL | 1× |
| NEBNext Q5 Hot Start HiFi Master Mix | 25μL | 1× |
| i7 Primer | 5μL | 1μM |
| i5 Primer | 5μL | 1μM |
| Total Volume | 50μL |
Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.