Genome-wide analysis of replication timing by next-generation sequencing with E/L Repli-seq

. Author manuscript; available in PMC: 2018 Jul 13.

Published in final edited form as: Nat Protoc. 2018 Mar 29;13(5):819–839. doi: 10.1038/nprot.2017.148

Component	Amount	Final concentration
Indexed library from Step 72	20μL	1×
TS-Oligo 1&2 (6μM each)	5μL	0.6μM each
NEBNext Q5 Hot Start HiFi Master Mix	25μL	1×
Total volume	50μL