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Change in UV thermal melting temperature (ΔTm) of the antiparallel GQ produced by OTAA upon site-specific incorporation of ThdG. Oligonucleotide solutions (6 μM) were monitored at 295 nm over five ramps at a rate of 0.5 °C min–1 in the OTA binding buffer. ΔTm = Tm (mOTAA GQ) – Tm (native OTAA GQ). Error bars represent the range in ΔTm due to differences in Tm values between the melting and annealing curves (hysteresis).
