Table 2. Effects of the Protein Density Immobilized on the Surface of Agarose Beads on the Adsorption of Antibody.
| adsorbent | IgG-binding (mg/g) | IgA-binding (mg/g) | IgM-binding (mg/g) | antibody-binding capacity (mg/g) | antibody-binding ratio (mol/mol)b |
|---|---|---|---|---|---|
| agarose beads | 1.6 | a | a | 1.6 | |
| A-SI-1 | 10.2 | a | a | 10.2 | 1.46 |
| A-SI-2 | 19.2 | 0.9 | a | 20.1 | 1.44 |
| A-SI-4 | 34.6 | 1.6 | 2.1 | 38.3 | 1.47 |
| A-SI-6 | 49.9 | 2.3 | 3.2 | 55.4 | 1.48 |
| A-SI-8 | 57.6 | 2.7 | 3.7 | 64 | 1.33 |
| A-SI-10 | 58 | 2.7 | 3.6 | 64.3 | 1.11 |
| A-RI-1 | 6.9 | a | a | 6.9 | 1.01 |
| A-RI-2 | 12.1 | a | a | 12.1 | 0.88 |
| A-RI-4 | 19.0 | 0.9 | a | 19.9 | 0.73 |
| A-RI-6 | 25.3 | 1.2 | 1.6 | 28.1 | 0.71 |
| A-RI-8 | 27.8 | 1.3 | 1.7 | 30.8 | 0.59 |
| A-RI-10 | 28.1 | 1.3 | 1.6 | 31 | 0.48 |
a
The data are not available because the concentration is too low to exceed the lowest limit of immunoturbidimetry measurement.
b
The antibody-binding ratio is calculated assuming that the antibody is composed of IgG because of the IgG purity of more than 90%.