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. 2017 Oct 19;2(10):6939–6957. doi: 10.1021/acsomega.7b00741

Figure 8.

Figure 8

Quantification of downregulation of mRNA levels of selected targeted proteins. MDA-MB-231 cells were treated with peptide/siRNA complexes with peptide/siRNA w/w ratios of 40:1 and 120:1 for linear and cyclic peptides, respectively, while the effect of incorporation of DOPE in the delivery system was also evaluated. Lipofectamine 2000 (Lipo.) was used as the positive control. The mRNA levels of KSP (A) and JAK2 (B) was quantified after 48 h of exposure to siRNA complexes using quantitative PCR. Data are presented as mean of RQ compared to cells exposed to saline (normalized using mRNA levels of β-actin), n = 3, and the error bars represent standard deviation. The asterisks identify the study groups that demonstrated a significant increase in silencing efficiency as a result of DOPE incorporation. DOPE (low) and (high) indicate the siRNA uptake of the cells exposed to DOPE (with no peptide) using the lowest and highest DOPE final concentrations used in the other study groups included in the experiment. (C) Correlation between silencing efficiency for the two selected targets: RQs presented in (A) and (B) are plotted on x and y axes, respectively, and the correlation factor (R) was calculated. The silencing data show significant correlation (p < 0.01) between the two selected targets.