Table 1. Primers Used in This Studya.
| gene | primer | description |
|---|---|---|
| ΔFAA1-F | 5′CAATAAAAACTAGAACAAACACAAAAGACAAAAAAAGACAACAATCAGCTGAAGCTTCGTACGC3′ | forward primer for FAA1 gene deletion |
| ΔFAA1-R | 5′TGCTTTAGTATGATGAGGCTTTCCTATCATGGAAATGTTGATCCAGCATAGGCCACTAGTGGATCTG3′ | reverse primer for FAA1 gene deletion |
| ΔFAA4-F | 5′TCTGTTCTTCACTATTTCTTGAAAAACTAAGAAGTACGCATCAAACAGCTGAAGCTTCGTACGC3′ | forward primer for FAA4 gene deletion |
| ΔFAA4-R | 5′GTGTTTATGAAGGGCAGGGGGGAAAGTAAAAAACTATGTCTTCCTGCATAGGCCACTAGTGGATCTG3′ | reverse primer for FAA4 gene deletion |
| ΔDGAI-F | 5′ATACATAAGGAAACGCAGAGGCATACAGTTTGAACAGTCACATAACAGCTGAAGCTTCGTACGC3′ | forward primer for DGAI gene deletion |
| ΔDGAI-R | 5′CCTTATTTATTCTAACATATTTTGTGTTTTCCAATGAATTCATTAGCATAGGCCACTAGTGGATCTG3′ | reverse primer for DGAI gene deletion |
| acot-F | 5′GAAGAAGCGGCCGCATGCTAGCCAAAGGTAAGCC3′ | forward primer for acot integrated in pMRI plasmid |
| acot-R | 5′CCTTAGATCTTCAAGGACTAGGTCTCTTGTCACC3′ | reverse primer for acot integrated in pMRI plasmid |
a
The car gene encoding CAR and sfp gene encoding SFP were artificially synthesized with SpeI/PacI and BamHI/XhoI endonuclease sites, respectively, and inserted into the pESC-leu plasmid directly.