Figure 1.

Droplet–ESI-MS methodology for high-throughput biocatalysts screening in Directed Evolution. Following the arrows: DNA templates of selected enzymes are created on plasmids; the cells are transformed with DNA-carrying plasmids; the cells are cultured and the target enzymes are produced; the cells are lysed to release the enzymes; enzymatic assays are carried out in multiwell plates; sample droplets are generated into a Teflon tube from the assay plates; sample droplets are infused into ESI-MS for high-throughput screening.