Table 2. In vitro radiolabeling results of aryl diamine-conjugated HSA 9 using [125I] 5.
| entrya | concentration of 9b (μmol) | time (h) | % RCYc |
|---|---|---|---|
| 1 | 50 | 0.5 | 54 |
| 2 | 50 | 1 | 63 |
| 3 | 50 | 2 | 94 |
| 4 | 25 | 2 | 89 |
| 5 | 5 | 2 | 67 |
Solvent: PBS, Cu+2, (0.1 equiv), 25 °C.
Final concentration in the reaction mixture.
Radiochemical yield determined by radio-TLC.