Figure 3.

SMFS on Ctta-dockerin-labeled 7× Titin-Ig and sfGFP. (A) Force distance traces showing complete unfolding of the POI (Titin-Ig unfolding is shown in the upper trace, sfGFP in the lower trace). (B) Transformation of traces from (A) into contour-length space. (C) Force histograms of complex dissociation events and unfolding events of the POI: the upper two panels contain data from the bulk-expressed proteins and the lower two panels contain data from in vitro-expressed proteins. C/D complex dissociation can occur with [as in both sample traces shown in (A)] or without prior unfolding of the x-module, which is a subdomain of the dockerin, resulting in two populations of the dissociation forces. Each population was fitted with the Bell–Evans model.²³