Table 1. Bead Probe Small Molecule and the Number of PKs Identified on Data Combined from 90 and 180 min LC–MS/MS Analyses of the Bead Pull-Down of a HeLa Lysate.
bead | number of PKs identifieda | number of PKs overlapping with blank beads |
---|---|---|
blocked beads (control) | 23 | NA |
BIMX (S1) | 40 | 20 |
CZC8004 (S2) | 61 | 19 |
purvalanol B (S6) | 65 | 17 |
sunitinib (S7) | 20 | 11 |
dasatinib (S8) | 64 | 19 |
CTx-0294885 (S9) | 105 | 21 |
KC19 (S10) | 100 | 22 |
Analyses were conducted as duplicates, using 90 and 180 min runs.