Figure 2.

Thermostability assay of the luciferase variants (A) WT, (B) LGR, (C) Mutant E, and (D) YY5. The purified luciferases were incubated at various temperatures (25–45 °C) and sampled over time. Then, the samples were assayed for enzyme activity at 25 °C. The experiments were performed in triplicate, and the error bars indicate standard error.