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. 2018 Mar 6;3(3):2628–2633. doi: 10.1021/acsomega.7b02068

Table 1. Kinetic Parameters of Firefly Luciferase Variantsa.

enzyme Km (μM) kcat (cps/nM) kcat/Km
WT 1.21 ± 0.04 9.70 × 104 8.00 × 104
LGR 0.76 ± 0.06 3.87 × 105 5.09 × 105
mutant E 8.81 ± 0.27 1.05 × 106 1.20 × 105
YY5 4.20 ± 0.18 1.37 × 106 3.26 × 105
a

Apparent kcat values were obtained by dividing the calculated apparent Vmax(cps) by the luciferase concentration (7 nM).