Figure 3.

Transfection and cellular enhanced green fluorescent protein (eGFP) expression studies with PS-Lips and P-Lip. Comparative in vitro gene-delivery efficiencies of cationic lipid nanocarrier systems, PS-Lips and P-Lip in complexation with pCMV-SPORT-β-gal plasmid DNA using the reporter gene assay in multiple cultured cell lines at a 2:1 lipid/DNA charge ratio (A). Microscopy images for cellular eGFP expression in human embryonic kidney (HEK-293) cells (B). Lipoplexes of PS-Lips and P-Lip with pDNA encoding GFP were transfected in HEK-293 cells at a 2:1 lipid/DNA charge ratio, and images were obtained using an inverted fluorescence microscope. Lipofectamine 2000 was used as the positive control for transfections.