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| gene | sequence (5′−3′) | annealing temperature (°C) |
|---|---|---|
| c-MYC | F-TCTCCACACATCAGCACAAC (20 bp) | 51.8 |
| R-TCCGTTTTAGCTCGTTCCTC (20 bp) | 51.8 | |
| GAPDH | F-GATGCTGGCGCTGAGTACGTCGTG (24 bp) | 62.5 |
| R-AGTGATGGCATGGACTGTGGTCATGAG (24 bp) | 61.3 |
