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. 2018 Mar 6;3(3):2690–2698. doi: 10.1021/acsomega.7b01808

Figure 1.

Figure 1

GPC elution profiles of cellulose tricarbanilates. Cellulose samples (as labeled in the figure) were carbanilated, as described in Materials and Methods. For cellulose synthesized from BcsA-BcsB or AcsA-AcsB, 0.5 μM enzyme with 5 mM UDP-Glc in a reaction volume of 1.0 mL was used, as described in Materials and Methods. Reactions were allowed to proceed for 12 h. A KD-806M column was used with tetrahydrofuran as the mobile phase at a flow rate of 0.5 mL min–1. The figure shows the molecular weight distribution, converted to DOP on the right axis, above the associated refractive index detection signal.