Table 2. Quantitative Values of Zone of Inhibition against E. coli, MIC Calculation, and OD600 Value Measurements after 18 h Incubation of Polymer-Treated Cells.
| radius of zone of inhibition (cm) (R1) |
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| polymer | volume (μL) | set 1 | set 2 | SDa | R1 (average) | zone of inhibition (cm2)b | MIC (μg/mL) | MIC (μM) | concentration (μg/mL) | OD600 |
| DLP | 5 | 0.00 | 0.00 | 0 | 0.00 | 0.00 | ∼80 | ∼1.6 | 200 | 0.358 |
| 10 | 0.44 | 0.46 | 0.014 | 0.45 | 0.51 | 400 | 0.199 | |||
| 20 | 0.55 | 0.55 | 0 | 0.55 | 0.82 | 600 | 0.205 | |||
| DHBP1 | 5 | 0.00 | 0.00 | 0 | 0.00 | 0.00 | NCc | NCc | NC | NC |
| 10 | 0.41 | 0.39 | 0.014 | 0.40 | 0.38 | NC | NC | |||
| 20 | 0.58 | 0.62 | 0.028 | 0.60 | 1.00 | NC | NC | |||
| DHBP2 | 5 | 0.00 | 0.00 | 0 | 0.00 | 0.00 | NC | NC | NC | NC |
| 10 | 0.43 | 0.37 | 0.042 | 0.40 | 0.38 | NC | NC | |||
| 20 | 0.62 | 0.58 | 0.028 | 0.60 | 1.00 | NC | NC | |||
| DHBP3 | 5 | 0.00 | 0.00 | 0 | 0.00 | 0.00 | 50 | 1.4 | 200 | 0.175 |
| 10 | 0.00 | 0.00 | 0 | 0.00 | 0.00 | 400 | 0.271 | |||
| 20 | 0.48 | 0.52 | 0.028 | 0.50 | 0.66 | 600 | 0.207 | |||
| DHBP4 | 5 | 0.00 | 0.00 | 0 | 0.00 | 0.00 | NC | NC | 200 | 0.315 |
| 10 | 0.43 | 0.37 | 0.042 | 0.40 | 0.38 | 400 | 0.344 | |||
| 20 | 0.68 | 0.72 | 0.028 | 0.70 | 1.41 | 600 | 0.351 | |||
| DSP1 | 5 | 0.00 | 0.00 | 0 | 0.00 | 0.00 | NC | NC | NC | NC |
| 10 | 0.43 | 0.47 | 0.028 | 0.45 | 0.51 | NC | NC | |||
| 20 | 0.50 | 0.50 | 0 | 0.50 | 0.66 | NC | NC | |||
| DSP2 | 5 | 0.00 | 0.00 | 0 | 0.00 | 0.00 | NC | NC | NC | NC |
| 10 | 0.48 | 0.42 | 0.042 | 0.45 | 0.51 | NC | NC | |||
| 20 | 0.60 | 0.60 | 0 | 0.60 | 1.00 | NC | NC | |||
| DSP3 | 5 | 0.00 | 0.00 | 0 | 0.00 | 0.00 | NC | NC | NC | NC |
| 10 | 0.39 | 0.41 | 0.014 | 0.40 | 0.38 | NC | NC | |||
| 20 | 0.55 | 0.55 | 0 | 0.55 | 0.82 | NC | NC | |||
| DSP4 | 5 | 0.00 | 0.00 | 0 | 0.00 | 0.00 | NC | NC | 200 | 0.379 |
| 10 | 0.29 | 0.31 | 0.014 | 0.30 | 0.16 | 400 | 0.379 | |||
| 20 | 0.52 | 0.48 | 0.028 | 0.50 | 0.66 | 600 | 0.337 | |||
| DSP5 | 5 | 0.00 | 0.00 | 0 | 0.00 | 0.00 | 50 | 1.2 | 200 | 0.075 |
| 10 | 0.42 | 0.38 | 0.028 | 0.40 | 0.38 | 400 | 0.109 | |||
| 20 | 0.55 | 0.55 | 0 | 0.55 | 0.82 | 600 | 0.140 | |||
Standard deviation.
Zone of inhibition = π(R12 – r2), r = radius of sterilized filter paper = 0.2 cm.
Not calculated.