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. 2018 Jan 8;3(1):208–217. doi: 10.1021/acsomega.7b01657

Figure 2.

Figure 2

Protection assay of pDNA against degradation by DNase I (gel electrophoresis experiment): (a) pCMV-Luc plasmid, (b) pEGFP-C3 plasmid, and (c) a visual guide. In both experiments: lane 1, pDNA; lane 2, pDNA–DNase I; and lanes 3–6, C6C22C6/DOPE–pDNA lipoplexes at different molar compositions of the cationic lipid in the mixed lipids (α = 0.2 in lanes 3–4 and α = 0.5 in lanes 5–6) and different effective charge ratios of the lipoplex, ρeff = 4 (lanes 3 and 5) and ρeff = 10 (lanes 4 and 6).