Figure 4.

Cell viability of COS-7 cells in the presence of C₆C₂₂C₆/DOPE–pDNA lipoplexes at two molar compositions of the cationic lipid in the mixed lipids (α = 0.2 and 0.5). The plasmids used were (a) pEGFP-C3 and (b) pCMV-Luc. The experiments were performed with 10% serum (FBS). The orange and green bars correspond to the effective charge ratios of ρ_eff = 4 and 10 in the lipoplex, respectively. The gray bar corresponds to Lipo2000*, used here as the positive control. The results were normalized to those obtained for untreated cells (100%).