. 2017 Sep 15;2(9):5813–5823. doi: 10.1021/acsomega.7b01158

Table 3. Calculated Trehalose Concentrations and Percent Recoveries from Trehalose Samples Prepared in E. coli DH5α Cell Lysates or 1:1 E. coli DH5α Cell Lysates/Acetonitrile for All Three Assays.

assay	expected [trehalose]	calculated [trehalose]^a	percent recovery
enzymatic trehalose assay	30 μM	29 ± 2 μM	97
	80 μM	79 ± 4 μM	99
	250 μM	240 ± 20 μM	96
	800 μM	792 ± 5 μM	99
HPLC-RID	5 mM	4.77 ± 0.05 mM	95
	40 mM	38.5 ± 0.4 mM	96
	80 mM	84.4 ± 0.7 mM	106
LC–MS/MS	5 μM	5.0 ± 0.2 μM	100
	30 μM	28.3 ± 0.8 μM	94
	80 μM	74 ± 2 μM	93

n = 3–5.