Table 2. Binding Affinities (K_D) of SYNZIP Constructs As Determined by SPR.

	immobilized
in solution	D-1, nM	D-2, nM	D-3, nM	D-4, nM	D-1-3, nM	D-3-1, nM
A-1	–a	1.5	–	–	–	–
A-2	16	–	–	–	26	28
A-3	–	–	–	34	–	–
A-4	–	–	11	–	74	39
1-A	–	5.5	–	–	–	–
3-A	–	–	–	17	–	–
4-A	–	–	29	–	135	63
A-1-A	–	0.9	–	–	–	–

^a“–” indicates no binding observed.