Table 4. Binding Affinities (KD) As Determined by SPR.
immobilized |
||||||
---|---|---|---|---|---|---|
in solution | D-5, nM | D-6, nM | D-17, nM | D-18, nM | D-R34m, nM | D-E34m, nM |
A-1 | –a | – | – | – | – | – |
A-2 | – | – | – | – | – | – |
A-3 | – | – | 15b | – | – | – |
A-4 | – | 37b | – | – | – | – |
A-5 | – | 8.4 | – | 327 | – | – |
A-6 | 36 | – | 34 000b | – | – | – |
A-17 | – | 33 | – | 1.5 | – | – |
A-18 | – | – | 12 | – | – | – |
A-R34m | – | – | – | 268 | – | 10 |
A-E34m | – | – | – | – | 12 | – |
“–” indicates no binding observed.
Low signal, RU (response units) less than 50.