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. 2017 Oct 23;2(10):7085–7095. doi: 10.1021/acsomega.7b01234

Figure 2.

Figure 2

Surface coatings: (a) representative CLSM images, taken at different focal planes, of the coated Alg+ (i) and Algc (ii) particles cocultured with the HepG2 cells for 72 h (cell-to-particle ratio 25/1). The scale bars are 50 μm [blue: 6-diamidino-2-phenylindole (DAPI)-stained nuclei; green: fluorescently labeled Alg; and red: phalloidin-stained cytoskeleton]. (b) Cell viability: the viability of the HepG2 cells in the cocultures in comparison to that of a HepG2 monoculture is shown by assessing the activity of cellular dehydrogenase (culture time: 72 h, cell-to-Alg-based particle ratio: 25/1, and n = 3).