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Time courses of fluorescent intensity of bright spots corresponding to the binding and dissociation of single-molecule hybridization in (A) 8 mer–8 mer, (B) 12 mer–12 mer, and (C) dA12–dT12 DNAs. Time-lapse images were obtained at an interval of 30 s. Experimental conditions: 200 mM NaCl Tris-EDTA (TE) buffer (10 mM Tris–HCl, pH 8.0, 1 mM ethylenediaminetetraacetic acid (EDTA), 200 mM NaCl) [Cy3-DNA] = 1 nM at 20 °C.
