Table 3. Mismatch Discrimination by the L and R FIT-PNA Series.
mismatch | FIT-PNA |
|||||
---|---|---|---|---|---|---|
+3L | 0L | –3L | +3R | 0R | –3R | |
T:t | 0.98a | 0.90 | 0.81 | 0.62 | 0.70 | 0.75 |
C:t | 0.53 | 0.52 | 0.54 | 0.61 | 0.52 | 0.55 |
G:t | 0.72 | 0.75 | 0.73 | 0.41 | 0.33 | 0.40 |
A:c | 0.56 | 0.63 | 0.68 | 0.53 | 0.48 | 0.40 |
T:c | 0.86 | 0.74 | 0.80 | 0.48 | 0.45 | 0.56 |
C:c | 0.65 | 0.75 | 0.97 | 0.48 | 0.49 | 0.45 |
All values are relative to fully matched duplex (I/If). Lower case letters denote PNA nucleobases.