(A) Alignment of C. glutamicum homoserine
kinase (CglThrB) sequence with those of E. coli, M. jannaschii, and M. tuberculosis using CLUSTAL
O (1.2.4). Conserved residues are shown with asterisk (*), residues
with strongly similar properties are shown with a colon (:), and residues
with weakly similar properties are shown with a period (.). The conserved
Ala residues in motif I are highlighted in yellow. The arrows represent
β-strands, whereas the ovals represent α-helices. (B)
Structure of C. glutamicum ThrB (CglThrB-WT) with modeled l-homoserine and AMP-PNP
based on the structure of M. jannaschii ThrB, with these compounds bound in the active site.