| strain |
E. coli |
Top 10 |
E. coli host for molecular cloning |
Invitrogen |
| BL21 (DE3) |
E. coli host for protein
expression |
Invitrogen |
| C. glutamicum |
ATCC 13032 |
genomic DNA as a template for polymerase chain reaction (PCR) |
ATCC |
| plasmid |
pACYCDuet-1 |
duel expression vector (Cmr) |
Invitrogen |
| pCK448 |
pACYCDuet-1 carrying C. glutamicumthrB-6his under PT7
|
in house |
| pCK449 |
pACYCDuet-1 carrying C. glutamicumthrBA16G-6his under PT7
|
in house |
| pCK450 |
pACYCDuet-1 carrying C. glutamicumthrBA16V-6his, this study under PT7
|
in house |
| pCK453 |
pACYCDuet-1 carrying C. glutamicumthrBA16L-6his,
this study under PT7
|
in house |
| pCK454 |
pACYCDuet-1 carrying C. glutamicumthrBA16S-6his, this study under PT7
|
in house |
