Table 1. Parameters of Competitive Binding Analysis for HSA with MTX and FA at 298 K.
system | fixed [drug] (μM) | KI (M–1) | KII (M–1) | nI | nII |
---|---|---|---|---|---|
HSA–[FA]–MTX | 0 | (1.64 ± 0.11) × 105 | (0.16 ± 0.02) × 105 | 0.36 ± 0.04 | 2.21 ± 0.14 |
2 | (1.74 ± 0.14) × 105 | (0.24 ± 0.02) × 105 | 0.41 ± 0.05 | 1.73 ± 0.12 | |
10 | (0.78 ± 0.09) × 105 | (0.43 ± 0.03) × 105 | 0.58 ± 0.05 | 1.20 ± 0.09 | |
30 | (0.75 ± 0.06) × 105 | 0.56 ± 0.06 | |||
HSA–[MTX]–FA | 0 | (0.87 ± 0.08) × 105 | 1.02 ± 0.07 | ||
2 | (0.79 ± 0.07) × 105 | 1.04 ± 0.06 | |||
10 | (0.74 ± 0.09) × 105 | 1.12 ± 0.07 | |||
30 | (0.71 ± 0.07) × 105 | 1.01 ± 0.05 |