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. Author manuscript; available in PMC: 2019 Aug 1.
Published in final edited form as: Biotechnol Bioeng. 2018 May 14;115(8):2075–2086. doi: 10.1002/bit.26721

Figure 3. Egg Retrieval from Encapsulated Follicles in Alginate Hydrogels in Subcutaneous Site 7 Days Post-Transplant and Their Meiotic Maturation In Vitro.

Figure 3

(A) Follicles were easily identified in explanted hydrogels. (B) MII oocytes were confirmed via polar body extrusion (indicated with black arrows). (C) Egg retrieval and MII status from the subcutaneous site. Of the GV oocytes collected from antral follicles in Day 7 explants with 1,100 ovarian follicles, 34/54 oocytes were MII post-in vitro maturation (63% MII rate) from 3 trials. Oocytes collected from Day 7 explants with 730 or 360 ovarian follicles resulted in an MII rate of 55% (6 MII oocytes/11 GV oocytes) and 46% (10 MII oocytes/22 GV oocytes collected), respectively (D) Normal spindle morphology (indicated by an arrow) was also confirmed in subcutaneously-matured oocytes. Image (D) depicts an oocyte obtained from an explant containing ovarian follicles from 3 ovaries. Scale bar: 100 μm (A, B).