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. Author manuscript; available in PMC: 2019 Aug 1.
Published in final edited form as: Biotechnol Bioeng. 2018 May 14;115(8):2075–2086. doi: 10.1002/bit.26721

Figure 4. Egg Retrieval from Encapsulated Follicles in Alginate Hydrogels in Bursa Site 7 Day Post-Transplant and Their Meiotic Maturation In Vitro.

Figure 4

(A) Germinal vesicle (GV) oocytes were retrieved from antral follicles (B) MII oocytes were confirmed via polar body extrusion (indicated with black arrows) (C) Egg retrieval and MII status from the bursa site. Of the 15 GV oocytes collected at Day 7 from 1 trial, 3 were MII post-IVM (20% MII rate). Note: MII follicles were only observed with the 1,100 follicle condition and not for the 730 or 360 follicle condition. (D) Normal spindle morphology (indicated by an arrow) was also confirmed in MII oocytes. Image (D) depicts an oocyte obtained from an explant containing ovarian follicles from 3 ovaries. Scale bar: 100 μm (A, B).