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Protein solubility measured in three steps via SE-HPLC in unprocessed glutenin (powders) to observe the extent of depolymerization. LMW: low molecular weight; HMW: high molecular weight; AG: aggregated glutenin; 1Ex: SDS–phosphate buffer; 2Ex: SDS–phosphate buffer + 30 s sonication; 3Ex: SDS–phosphate buffer + 30 + 60 + 60 s sonication. The error bars represent standard deviations.
