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. 2018 May 24;3(5):5584–5592. doi: 10.1021/acsomega.7b02081

Figure 5.

Figure 5

Protein solubility in glutenin films. (a) Protein size distribution and solubility measured by SE-HPLC. LMW: low molecular weight; HMW: high molecular weight; AG: aggregated glutenin; 1Ex: SDS–phosphate buffer; 2Ex: SDS–phosphate buffer + 30 s sonication; 3Ex: SDS–phosphate buffer + 30 + 60 + 60 s sonication. (b) Protein extractability measured by RP-HPLC in different solvents: (1) 70% ethanol, (2) 50% propanol, (3) 50% propanol, 60 °C, (4) 50% propanol 0.5% SDS, 60 °C, (5) 50% propanol 1% dithiothreitol (DTT), 60 °C, and (6) 1% DTT, 1% SDS, 6 M urea solution, 100 °C. Protein solubility was normalized to average total solubility of all unprocessed glutenin samples. The error bars represent standard deviations.